Regents Professor, Department of Horticulture, Ph.D.1970, Utah State University.
This laboratory has a long-standing interest in understanding the Ca2+/calmodulin-mediated signaling in plants. Calmodulin (CaM), a ubiquitous and multifunctional Ca2+-binding protein, is a primary intracellular Ca2+ receptor, which transduces the second messenger Ca2+ signal by binding to and altering the activity of the other proteins. To study the downstream elements involved in Ca2+/CaM-mediated stress signal transduction, 35S-labeled CaM was used for protein-protein interaction-based screening of several expression libraries.
By this approach, we have cloned and characterized several genes that encode for CaM-binding proteins. The primary goal of this research is to investigate the functional significance of these genes, and understand how they are involved in Ca2+/CaM-mediated signaling. Ca2+/CaM-regulated protein phosphorylation is believed to play a pivotal role in amplifying and diversifying the action of Ca2+-mediated signals. This laboratory has cloned a chimeric Ca2+/CaM-dependent protein kinase (CCaMK) gene.
CCaMK is characterized by the presence of a kinase domain, an autoinhibitory domain, a CaM-binding domain and a neural visinin-like Ca2+-binding domain in a single polypeptide. This laboratory has also cloned a Ca2+/CaM-dependent receptor-like kinase, and the role of this and other CaM-binding proteins are being investigated. Image
Qiu, Y., Xi, J., Du, L. and Poovaiah, B.W. 2012. The function of calreticulin in plant immunity: New discoveries for an old protein. Plant Signaling & Behavior 7:1-4.
Qiu, Y., Xi, J., Du, L., Suttle, J. and Poovaiah, B.W. 2012. Coupling calcium/calmodulin-mediated signaling and herbivore-induced plant response through calmodulin-binding transcription factor AtSR1/CAMTA3. Plant Mol. Biol. 79:89-99 (with journal cover page).
Xi, J., Qiu, Y., Du, L. and Poovaiah, B.W. 2012. Plant-specific trihelix transcription factor AtGT2L interacts with calcium/calmodulin and responds to cold and salt stresses. Plant Sci. 185-186:274-280.
Qiu, Y., Xi, J., Du, L., Roje, S. and Poovaiah, B.W. 2012. A dual regulatory role of Arabidopsis calreticulin 2 in plant innate immunity. Plant J. 69(3):489–500.
Yang, T., Ali, G.S., Yang, L, Du, L., Reddy, A.S.N., Poovaiah, B.W. 2010. Calcium/calmodulin-regulated Receptor-like Kinase CRLK1 interacts with MEKK1 in Plants. Plant Signaling and Behavior 5:991-994.
Yang, T, Chaudhuri, S, Yang, L, Du, L, Poovaiah, B.W. 2010. A calcium/calmodulin-regulated member of the receptor-like kinase family confers cold tolerance in plants. J. Biol. Chem. 285:7119-7126.
Du, L., Ali, G. S., Simons, K. A., Hou J., Yang, T., Reddy, A.S.N. and Poovaiah, B. W. 2009. Ca2+/calmodulin regulates salicylic-acid-mediated plant immunity. Nature 457:1154-1158.
Yang, T., Du, L. and Poovaiah, B.W. 2007. Concept of redesigning proteins by manipulating calcium/calmodulin-binding domains to engineer plants with altered traits. Functional Plant Biology 34:343-352.
Gleason, C., Chaudhuri, S., Yang, T., Munoz-Gutierrez, A., Poovaiah, B.W. and Oldroyd, G.E.D. 2006. Nodulation independent of rhizobia induced by a calcium-activated kinase lacking autoinhibition. Nature. 441:1149-1152.
Du, L. and Poovaiah, B. W. 2005. Calcium/calmodulin is critical for brassinosteroid biosynthesis and plant growth. Nature. 437:741-745.
U.S. patents: No. 5, 498, 533 (1996); No. 6, 077, 991 (2000); No. 6, 362, 395 (2002) and No. 6, 403, 352 (2002).