WSU Regents Professor, Department of Horticulture
Plants are constantly exposed to external and internal stimuli (signals). They adapt by sensing these signals and responding to them. These external and internal stimuli are communicated by chemical messengers. One of the most important chemical signals used by plants is the calcium ion (Ca2+). This laboratory has a long-standing interest in understanding Ca2+/calmodulin-mediated signaling in plants. Calmodulin (CaM), a ubiquitous and multifunctional Ca2+-binding protein, is the primary intracellular Ca2+ receptor, which transduces the second messenger Ca2+ signal by binding to and altering the activity of other proteins. We have cloned and characterized a number of genes that encode for CaM-binding proteins and studied their role in Ca2+/CaM-mediated signaling. The primary goal of this research is to investigate the functional significance of these genes, with an emphasis on plant growth and plant-microbe interactions.
Poovaiah, B. W. and Leopold, A. C. 1973. Deferral of leaf senescence with calcium. Plant Physiol. 52:236-239.
Poovaiah, B. W. and Leopold, A. C. 1973. Inhibition of abscission by calcium. Plant Physiol. 51:848-851.
Poovaiah, B. W. and Rasmussen, H. P. 1973. Calcium distribution in the abscission zone of bean leaves. Electron microprobe x-ray analysis. Plant Physiol. 52:683-684.
Veluthambi, K. and Poovaiah, B. W. 1984. Calcium-promoted protein phosphorylation in plants. Science 233:167-169.
Paliyath, G. and Poovaiah, B. W. 1984. Calmodulin inhibitor in senescing apples and its physiological and pharmacological significance. Proc. Natl. Acad. Sci. USA 81:2065-2069.
Veluthambi, K. and Poovaiah, B. W. 1984. Calcium- and calmodulin-regulated phosphorylation of soluble and membrane proteins from corn coleoptiles. Plant Physiol. 76:359-365.
Poovaiah, B. W. and Reddy, A. S. N. 1987. Calcium messenger system in plants. CRC Crit. Rev. Plant Sci. 6:47-103.
McFadden, J. J. and Poovaiah, B. W. 1988. Rapid changes in protein phosphorylation associated with light-induced gravity perception in corn roots. Plant Physiol. 86:332-334.
Jena, P. K., Reddy, A. S. N. and Poovaiah, B. W. 1989. Molecular cloning and sequencing of a cDNA for plant calmodulin: Signal-induced changes in the expression of calmodulin. Proc. Natl. Acad. Sci. USA 86:3644-3648.
Patil, S., Takezawa, D. and Poovaiah, B.W. 1995. Chimeric plant calcium/calmodulin-dependent protein kinase gene with a neural visinin-like calcium-binding domain. Proc. Natl. Acad. Sci. 92: 4897-4901.
Takezawa, D., Ramachandiran, S., Paranjape, V. and Poovaiah, B.W. 1996. Dual regulation of a chimeric plant serine/threonine kinase by calcium and calcium/calmodulin. J. Biol. Chem. 271: 8126-8132.
Ramachandiran, S., Takezawa, D., Wang, W., and Poovaiah, B.W. 1997. Functional domains of plant chimeric calcium/calmodulin-dependent protein kinase: regulation by autoinhibitory and visinin-like domains. J. Biochem. 121: 984-990.
Poovaiah, B.W., Xia, M., Liu, Z., Wang, W., Yang, T., Sathyanarayanan, P.V. and Franceschi, V.R. 1999. Developmental regulation of chimeric calcium/calmodulin‑dependent protein kinase gene in anthers. Planta 209: 161-171.
Yang, T. and Poovaiah, B.W. 2000. An early ethylene up-regulated gene encoding a calmodulin-binding protein involved in plant senescence and death. J. Biol. Chem. 275: 38467-38473.
Sathyanarayanan, P.V., Cremo, C.R. and Poovaiah, B.W. 2000. Plant chimeric Ca2+/calmodulin-dependent protein kinase: Role of the neural visinin-like domain in regulating autophosphorylation and calmodulin affinity. J. Biol. Chem. 275: 30417-30422.
Sathyanarayanan, P.V., William F. Siems, Jeffrey P. Jones and B.W. Poovaiah. 2001. Calcium-stimulated autophosphorylation site of plant chimeric calcium/calmodulin dependent protein kinase. J. Biol. Chem. 276: 32940-32947.
Yang, T. and Poovaiah, B.W. 2002. Hydrogen peroxide homeostasis: Activation of plant catalase by calcium/calmodulin. Proc. Natl. Acad. Sci. 99: 4097-4102.
Sathyanarayanan, P.V. and B.W. Poovaiah. 2002. Autophosphorylation-dependent inactivation of plant chimeric calcium/calmodulin-dependent protein kinase. Eur. J. Biochem. 269: 2457-2463.
Yang, T. and Poovaiah, B. W. 2002. A Calmodulin-binding CGCG box DNA-binding protein family involved in multiple signaling pathways in plants. J. Biol. Chem. 277: 45049-45058.
Yang, T. and Poovaiah, B.W. 2003. Calcium/calmodulin-mediated signal network in plants. Trends in Plant Sci. 8:505-512.
Yang, T., Chaudhuri, S., Yang, L., Chen, Y., and Poovaiah, B. W. 2004. Calcium/calmodulin upregulates a cytoplasmic receptor-like kinase. J. Biol. Chem. 279: 42552-42559.
Du, L. and Poovaiah, B. W. 2004. A novel family of Ca2+/calmodulin-binding proteins involved in transcriptional regulation: Interaction with fsh/Ring3 class transcription activators. Plant Mol. Biol. 54: 549-569.
Du, L. and Poovaiah, B. W. 2005. Calcium/calmodulin is critical for brassinosteroid biosynthesis and plant growth. Nature. 437:741-745.
Gleason, C., Chaudhuri, S., Yang, T., Munoz-Gutierrez, A., Poovaiah, B.W. and Oldroyd, G.E.D. 2006. Nodulation independent of rhizobia induced by a calcium-activated kinase lacking autoinhibition. Nature. 441:1149-1152.
Du, L., Ali, G. S., Simons, K. A., Hou J., Yang, T., Reddy, A.S.N. and Poovaiah, B. W. 2009. Ca2+/calmodulin regulates salicylic-acid-mediated plant immunity. Nature 457:1154-1158.
Yang, T, Chaudhuri, S, Yang, L, Du, L, Poovaiah, B.W. 2010. A calcium/calmodulin-regulated member of the receptor-like kinase family confers cold tolerance in plants. J. Biol. Chem. 285:7119-7126.
Yang, T., Ali, G.S., Yang, L, Du, L., Reddy, A.S.N., Poovaiah, B.W. 2010. Calcium/calmodulin-regulated Receptor-like Kinase CRLK1 interacts with MEKK1 in Plants. Plant Signaling and Behavior 5:991-994.
Qiu, Y., Xi, J., Du, L., Roje, S. and Poovaiah, B.W. 2012. A dual regulatory role of Arabidopsis calreticulin 2 in plant innate immunity. Plant J. 69(3):489–500.
Qiu, Y., Xi, J., Du, L., Suttle, J. and Poovaiah, B.W. 2012. Coupling calcium/calmodulin-mediated signaling and herbivore-induced plant response through calmodulin-binding transcription factor AtSR1/CAMTA3. Plant Mol. Biol. 79:89-99.
Routray, P., Miller, J.B., Du, L., Oldroyd, G. and Poovaiah, B.W. 2013. Phosphorylation of S344 in the calmodulin-binding domain negatively affects CCaMK function during bacterial and fungal symbioses. Plant Journal, 76:287-296.
Poovaiah, B.W., Du, L., Wang, H. and Yang, T. 2013. Recent Advances in Calcium/Calmodulin-Mediated Signaling with an Emphasis on Plant-Microbe Interactions. Plant Physiology 163:531-542.
Zhang, L., Du, L., Shen, C., Yang, Y. and Poovaiah, B.W. 2014. Regulating Plant Immunity through Ubiquitin-mediated Modulation of Ca2+-Calmodulin-AtSR1/CAMTA3 Signaling. Plant Journal, 78:268-281.
Zeng, H., Xu, L., Singh, A., Wang, H., Du, L. and Poovaiah, B.W. 2015. Involvement of calmodulin and calmodulin-like proteins in plant responses to abiotic stresses. Front. Plant Sci., http://dx.doi.org/10.3389/fpls.2015.00600.
Yuan, P., Jauregui, E., Du, L., Tanaka, K. and Poovaiah, B.W. 2017. Calcium signatures and signaling events orchestrate plant-microbe interactions. Curr. Opin. in Plant Biol. 38:173-183.
Jauregui, E., Du, L., Gleason, C. and Poovaiah, B.W. 2017. Altered calmodulin-binding capacity of calcium/calmodulin-dependent protein kinase (CCaMK) negatively affects root nodule symbiosis in Medicago truncatula. Frontiers in Plant Sci. doi.org/10.3389/fpls.2017.01921.
Poovaiah, B.W. and Du, Liqun. 2018. Calcium signaling: decoding mechanism of calcium signatures. New Phytologist. 217:1598-1609.
Yuan, P., Tanaka, K., Du, L. and Poovaiah B.W. 2018. Calcium signaling in plant autoimmunity: A guard model for AtSR1/CAMTA3-mediated immune response. Molecular Plant 11, 637-639.
Yuan, P., Du, L. and Poovaiah, B.W. 2018. Ca2+/Calmodulin-Dependent AtSR1/CAMTA3 Plays Critical Roles in Balancing Plant Growth and Immunity. Int. J. Mol. Sci. 2018, 19(6), 1764; https://doi.org/10.3390/ijms19061764