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Washington State University Molecular Plant Sciences

509-335-3304
kirchhh@wsu.edu

Assistant Professor, Institute of Biological Chemistry, Ph.D. 1998, University of Munster, Germany

Kirchhoff

Research

Plants are integrated in a complex environment that fluctuate both randomly and periodically on very different time scales. Photosynthetic energy conversion must compensate for these changes to maintain energetic homeostasis for the cell. Failure to do this results either in reduced performance of energy transformation and consequently in a decrease in yield and fitness of plants or in severe damage by toxic photosynthetic side products that eventually lead to cell death. These potential problems are tackled by a battery of highly regulated optimization, protection, and repair mechanisms. Most of these mechanisms are realized in the photosynthetic thylakoid membranes that harbor the sophisticated, structured nanomachines responsible for biological energy conversion. Our research aims to understand the mechanisms that optimize, protect, and maintain the photosynthetic machinery on the molecular and supramolecular level. Accomplishment of these ambitious aims will lead to insights on how plants survive in an challenging environment and can help to find new strategies to solve our global food and energy problems.
http://www.wix.com/yarbrord/kirchhoff-lab

Selected Publications

Peer Reviewed Articles:

H. Kirchhoff, Architectural switches in plant thylakoid membranes. Photosynthesis Research, accepted

C.P. Holleboom, S. Yoo, P.N. Lia, I. Compton, W. Haase, H. Kirchhoff, P.J. Walla, Carotenoid-chlorophyll coupling and fluorescence quenching correlates with protein packing density in grana-thylakoids. Journal of Physical Chemistry B, 2013, in press, Kirchhoff and Walls co-corresponding authors.

H. Kirchhoff (2013) Structural constraints for protein repair in plant photosynthetic membranes. Plant Signaling and Behavior, doi:pii: e23634.

H. Kirchhoff, R.M. Sharpe, M. Herbstova, R.Yarbrough, G.E. Edwards (2012) Differential mobility of pigment protein complexes in granal and agranal thylakoid membranes of C3 and C4 plants. Plant Physiology, 161, 497-507.

M. Herbstova, S. Tietz, C. Kinzel, M.V. Turkina, H. Kirchhoff (2012) Architectural switch in plant photosynthetic membranes induced by light stress. Proc. Natl. Acad. Sci. USA, 109, 20130-20135.

H. Kirchhoff, C. Hall, M. Wood, M. Herbstová, O. Tsabari, R. Nevo, D. Charuvi, E. Shimoni, Z. Reich (2011) Dynamic control of protein diffusion within the granal thylakoid lumen. Proc. Natl. Acad. Sci. USA, 108,20248-20253 (recommended by the Faculty of 1000).

S. Haferkamp, W. Haase, A.A. Pascal, H. van Amerongen, H. Kirchhoff (2010) Efficient light harvesting by photosystem II requires an optimized protein packing density in grana thylakoids. Journal Biological Chemistry 285, 17020-17028.

T. K. Goral, M. P. Johnson, H. Kirchhoff, A. V. Ruban, C.W. Mullineaux (2010) Visualizing the diffusion of chlorophyll-proteins in higher plant thylakoid membranes: Effects of photoinhibition and protein phosphorylation. The Plant Journal 62, 948-959.

H. Kirchhoff (2008) Significance of protein crowding, order and mobility for photosynthetic functions. Biochem. Soc.Transactions 36, 967-970.

H. Kirchhoff (2008) Molecular crowding and order in photosynthetic membranes. Trends in Plant Science 13, 201-207.

H. Kirchhoff, S. Haferkamp, J. F. Allen, D. Epstein, C. W. Mullineaux (2008) Significance of macromolecular crowding for protein diffusion in thylakoid membranes of chloroplasts. Plant Physiology 146, 1571-1578.

H. Kirchhoff, S. Lenhert, C. Büchel, L. Chi, J. Nield (2008) Probing the organization of photosystem II in photosynthetic membranes by atomic force microscopy. Biochemistry 47, 431-440.

S. Haferkamp, H. Kirchhoff (2008) Significance of molecular crowding in grana membranes of higher plants for light harvesting by photosystem II. Photosynthesis Research 95, 129-134.

B. Naumann, A. Busch, J. Allmer, E. Ostendorf, M. Zeller, H. Kirchhoff, M. Hippler (2007) Comparative quantitative proteomics to investigate the remodelling of bioenergetic pathways under iron-deficiency in Chlamydomonas reinharditi. Proteomics 7, 3964-3979.

H. Kirchhoff, W. Haase, S. Wegner, R. Daniellsson, R. Ackermann, P.-A. Albertsson (2007) Low-light induced array formation of photosystem II in higher plant chloroplasts. Biochemistry 46, 11169-11176.

H. Kirchhoff, W. Haase, S. Haferkamp, T. Schott, M. Borinski, U. Kubitscheck, M.Rögner (2007) Structural and functional self-organization of Photosystem II in grana thylakoids. Biochimica et Biophysica Acta-Bioenergetics 1767, 1180-1188.

T. Volkmer, D. Schneider, G. Bernat, H. Kirchhoff, S.-O. Wenk, M. Rögner (2006) SSR2998 of Synechocystis so. PCC 6803 is involved in regulation of cyanobacterial electron transport and associated with the cytochrome b6f complex. Journal Biological Chemistry 282, 3730-3737.

M.A. Schöttler, H. Kirchhoff, E. Weis (2004) The role of plastocyanin in the adjustment of the photosynthetic electron transport to the carbon metabolism in tobacco. Plant Physiology 136, 4265-4274.

H. Kirchhoff, M. Borinski, S. Lenhert, L. Chi, C. Büchel (2004) Transversal and lateral exciton energy transfer in grana thylakoids of spinach. Biochemistry 43, 14508-14516.

H. Kirchhoff, M.A. Schöttler, E. Weis (2004) Plastocyanin redox kinetics in chloroplasts: Evidence for a dis-equilibirium in the high potential chain. Biochimica et Biophysica Acta-Bioenergetics 1659, 63-72.

H. Kirchhoff, I. Tremmel, W. Haase, U. Kubitscheck (2004) Supramolecular photosystem II organization in grana thylakoid membranes: Evidence for a structured arrangement. Biochemistry 43, 9204-9213.

I. Tremmel, H. Kirchhoff, E. Weis, G.D. Farquhar (2003) Dependence of the plastoquinone diffusion coefficient on the shape, size, density of integral thylakoid proteins. Biochimica et Biophysica Acta-Bioenergetics 1607, 97-109.

H. Kirchhoff, H. J. Hinz, J. Rösgen (2003) Aggregation and chlorophyll a fluorescence quenching of the light harvesting complex II from spinach in vitro. Biochimica et Biophysica Acta-Bioenergetics 1606, 105-116.

M.A. Schöttler, H. Kirchhoff, K. Siebke, E. Weis (2002) Metabolic control of photosynthetic electron transport in CAM induced Mesembryantheum cristallinum L. Functional Plant Biology 29, 697-705.

H. Kirchhoff, U. Mukherjee, H.J. Galla (2002) The molecular architecture of the thylakoid membrane: the lipidic diffusion space for plastoquinone. Biochemistry 41, 4872-4882.

H. Kirchhoff, S. Horstmann, E. Weis (2000) Control of the photosynthetic electron transport by PQ diffusion microdomains in thylakoids of higher plants. Biochimica et Biophysica Acta-Bioenergetics 1459 (2000), 148-168.

Congress Abstracts:

S. Tietz, C. Kinzel, R. Yarbrough, H. Kirchhoff (2012) Significance of protein ordering in grana thylakoids for light-harvesting by photosystem II and protein mobility. In: Proceedings of 15th International Congress on Photosynthesis. Springer Press, in press.

H. Kirchhoff, S. Haferkamp, C. Mullineaux, H. van Amerongen (2009) Significance of protein order in thylakoid membranes for photosynthetic energy conversion. FEBS J. 276, 280-281.

S. Haferkamp, W. Haase, H. van Amerongen, H. Kirchhoff (2007) Molecular crowding and photosynthetic energy transformation. FEBS J. 274, 230-230.

H. Kirchhoff (2007) Protein diffusion and macromolecular crowding in grana. Photosyn. Res 91, 211-211.

H. Kirchhoff (2007) Probing the organization of photosystem II in photosynthetic membranes by atomic force microscopy. Photosyn. Res. 91215-216.

H. Kirchhoff, S. Haferkamp, J.F. Allen, C.W. Mullineaux (2007) Protein diffusion and macromolecular crowding in grana thylakoids. In: Proceedings of 14th International Congress on Photosynthesis. Springer Press.

M. Hippler, A. Busch, J. Allmer, E. Ostendorf, M. Zeller, H. Kirchhoff, B. Naumann (2007) Title: Comparative quantitative proteomics to investigate the remodelling of bioenergetic pathways under iron-deficiency in Chlamydomonas reinhardtii. Photosyn. Res. 91, 290-290.

S. Haferkamp, S. Wegner, W. Haase, H. Kirchhoff (2004) Dynamic interaction between LHCII and PSII in grana membranes fused with liposomes In: Proceedings of 13th International Congress on Photosynthesis. Allen Press Inc. Lawrence, Kansas (USA).

H. Kirchhoff, I. Tremmel, W. Haase, U. Kubitscheck (2004) Evidence for a structured protein organization in grana thylakoids. In: Proceedings of 13th International Congress on Photosynthesis. Allen Press Inc. Lawrence, Kansas (USA).

M.A. Schöttler, H.Kirchhoff, K.Siebke, E.Weis (2001) The photosynthetic electron transport in thylakoids of the facultative CAM plant Mesembryanthemum crystallinum (L.). In: PS2001 proceedings : 12th International Congress on Photosynthesis. CSIRO PUBLISHING, Australia, S18-003.

I. Tremmel, H. Kirchhoff, E. Weis, G.D. Farquhar (2001) Micro-organisation of thylakoids and the control of whole chain photosynthetic electron transport. In: PS2001 proceedings: 12th International Congress on Photosynthesis. CSIRO PUBLISHING, Australia, S18-004.

Book Chapters:

 

H. Kirchhoff (2012) Dynamic architecture of photosynthetic membranes. In: Advances in Plant Bioloty: Plastid Biology (Editors: F.-A. Wollman and S. Theg), Springer Press, in press.

C.W. Mullineaux, H. Kirchhoff (2009) Role of lipids in the dynamics of thylakoid membranes. In: Lipids in Photosynthesis: Essential and Regulatory Functions (Editors: H. Wada and N. Murata) Book Series: Advances in Photosynthesis and Respiration. Volume 30, 283-294, Springer Press.

C.W. Mullineaux, H. Kirchhoff (2007) Using fluorescence recovery after photobleaching to measure lipid diffusion in membranes. In: Methods in Molecular Biology (Editor: A.M. Dopico) 267-275, Humana Press (Totowa, NJ).

C. Yang, H. Kirchhoff, S. Boggasch, W. Haase, H. Paulsen (2005) Reconstitution of the major light-harvesting chlorophyll a/b complex into liposomes. In: Cell Biology Techniques (Editors: J.R. Harris, J. Graham,. D. Rickwood) 300-304, John Wiley & Sons, Ltd, London.