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Washington State University Molecular Plant Sciences

509-335-7545
tegeder@wsu.edu
Professor of Biological Sciences, Center for Reproductive Biology, Center for Integrated Biotechnology. Ph.D. Free University of Berlin and University of Goettingen.
tegeder

Research

Nitrogen is often a limiting factor to plant growth and development. Its distribution within the plant, mainly in the form of amino acids, is particularly important to generation of vegetative (tubers, bulbs, storage roots, etc.) and reproductive (flowers, seeds) organs. We are studying the transport of amino acids and other organic compounds, like ureides, from roots and leaves where they are produced to sink organs (like seeds) where they are essential for development and accumulation of storage compounds.

Several classes of putative organic nitrogen transporters have been identified in plants. Using molecular, biochemical, cell biological and physiological approaches we are currently characterizing the transporters and investigating the mechanisms of their regulation and physiological role in the model plant Arabidopsis as well as in crop plants such as the legumes (pea, French bean and soybean). The ultimate goal of my work is nutrient traffic control, i.e. manipulating nutrient flux and distribution within the plant body, targeted at improving plant productivity and nutritional quality of harvestable products.

Research areas include:

(a) Organic nitrogen acquisition from the soil;

(b) Mechanisms of assimilate partitioning, particularly amino acids and ureides;

(c) Flower and seed development with respect to nitrogen translocation processes;

(d) Source-sink interaction/phloem transport and regulation of nitrogen partitioning;

(e) Plant-bacteria symbiosis and fixation of atmospheric nitrogen;

(f) Nitrogen transport across subcellular membranes;

(f) Plant metabolism and metabolic network interaction;

(g) Importance of nitrogen partitioning in changing environments (global climate change);

(h) Relevance of nitrogen transport processes for improving plant biomass production and nutritional quality.

 

Representative Publications

Publications since 2011

Tegeder M., Rentsch D. and PatrickJ.W. (2011) Organic carbon and nitrogen transporters. In Murphy A., Peer W. and Schulz B., eds, Plant Plasma Membrane: Plant Cell Monographs, Springer, Berlin,pp 331-352.

Tegeder M. and Ward J.M. (2012) Molecular evolution of plant AAP and LHT amino acid transporters, Frontiers in Plant Physiology, 3, 1-11, doi: 10.3389/fpls.2012.00021

Tegeder M. (2012) Transporters for amino acids in plant cells: some functions and many unknowns. Current Opinion in Plant Biology, 15:315–321

Collier R. and Tegeder M. (2012) Soybean ureide transporters play a critical role in nodule development, function and nitrogen export. The Plant Journal 72, 355-367, COVER ILLUSTRATION.

Tegeder M., Ruan Y.-L. and Patrick J.W. (2013) Roles of membrane transporters in phloem functions; in Thompson G. and van Bel A., eds, Biochemistry of Phloem, Wiley-Blackwell, Oxford, pp 63-101.

 

Publications from 2006 -2010

Tegeder M. and Weber A.P.M. (2006) Metabolite transporters in control of primary plant metabolism. In: Control of primary Metabolism in plants. (Eds) W. C. Plaxton and M. T. McManus. Blackwell Publishing. 85-120.

Pélissier H.C. and Tegeder M. (2007) PvUPS1 plays a role in source-sink transport of allantoin in French bean (Phaseolus vulgaris). Functional Plant Biology, 34, 282-291.

Lee Y.H., Foster J., Chen J., Voll L.M., Weber A.P.M. and Tegeder M.(2007). AAP1 transports uncharged amino acids into roots of Arabidopsis. The Plant Journal, 50, 305-319.

Rentsch D., Schmidt S. and Tegeder M. (2007). Transporters for uptake and allocation of organic nitrogen compounds in plants. FEBS Letters, 581, 2281-2289.

Tegeder M., Tan Q., Grennan A. and Patrick J.W. (2007) Amino acid transporter expression and localisation studies in pea (Pisum sativum L.). Functional Plant Biology, 34, 1019-1028 (month’s feature paper).

Tan Q., Grennan A., Rentsch D. and Tegeder M. (2008) Isolation and characterization of an amino acid transporter from French bean (Phaseolus vulgaris L.). Plant Science 174, 348-356.

Foster J., Lee Y.H. and Tegeder M. (2008) Distinct expression of members of the LHT amino acid transporter family in flowers indicates specific roles in plant reproduction. Sex Plant Reprod 21143-152.

Komarova N.Y., Thor K., Gubler A., Meier S., Dietrich D., Weichert A., Suter Grotemeyer M., Tegeder M., and Rentsch D. (2008) AtPTR1 and AtPTR5 transport dipeptides in planta. Plant Physiol 148, 856-869.

Telang M.A., Giri A.P., Pyati P.S., Gupta V.S., Tegeder M. and Franceschi V.R. (2009) Winged bean chymotrypsin inhibitors retard growth of Helicoverpla anmigera. Gene 431, 80-85.

Sanders, A., R. Collier, A. Trethewy, G. Gould, R. Sieker and Tegeder M. (2009) AAP1 regulates import of amino acids into developing Arabidopsis embryos. Plant J 59, 540–552.

Tegeder M. and Rentsch D. (2010) Uptake and Partitioning of Amino Acids and Peptides. Molecular Plant 3, 997-1011.

Tan Q., Zhang L., GrantJ., CooperP. and Tegeder M. (2010) Altered phloem transport of S-methylmethionine affects plant metabolism and seed number in pea plants. Plant Physiology 154, 1886-1896.

Zhang L., Tan Q., Lee R., Trethewy A., Lee Y.-H. and Tegeder M. (2010) Altered amino acids transported by AAP2 changes seed yield and oil content in Arabidopsis. Plant Cell, 22, 3603-3620.

 

Publications from 2000 – 2005

Tegeder M., Thomas M., Hetherington L., Wang X.-D., Offler C.E. and Patrick J.W. (2000) Genotypic differences in seed growth rates of Phaseolus vulgaris L. Factors contributing to cotyledon sink activity and sink size. Aust J Plant Physiol 27, 119-29.

Tegeder M., Offler C.E., Frommer W.B. and Patrick J.W. (2000) Amino acid transporters are localized to transfer cells of developing pea seeds. Plant Physiol 122, 319-326.

Delrot S., Rochat C., Tegeder M. and Frommer W.B. (2001). Amino acid transport. In: Plant Nitrogen, (Eds) P Lea and JF Morot Gaudry, INRA-Springer, ISBN 3-540-67799-2, pp 213-235.

Rosche E., Blackmore D., TegederM., RichardsonT., SchroederH., HigginsT.J.V., Frommer W.B., Offler C.E. and Patrick J.W. (2002) Seed-specific overexpression of a potato sucrose transporter increases sucrose uptake and growth rates of developing pea cotyledons. Plant J 30 (2), 165-175.

DesimoneM., CatoniE., LudewigU., HilpertM., Schneider A., Kunze R., TegederM., FrommerW.B. and Schumacher K. (2002) A novel superfamily of transporters for allantoin and other oxo-derivatives of N heterocyclic compounds in Arabidopsis. Plant Cell 14, 847-56.

FischerW.N., LooD.D.F., KochW., Ludewig U., Boorer K.J., Tegeder M., Rentsch D., WrightE.M.and Frommer W.B. (2002) Low and high affinity amino acid H+-cotransporters for cellular import of neutral and charged amino acids. Plant J 29, 717-31.

Wipf D., Benjdia M., Tegeder M. and Frommer W.B.  (2002). Characterization of a general amino acid permease from Hebeloma cylindrosporum by functional complementation of a yeast mutant. FEBS Letters 528, 119-124.

Wipf D., Ludewig U., Tegeder M., Rentsch D., Koch W. and Frommer W.B. (2002) Amino acid/neurotransmitter transporters are highly conserved between fungi, plants, and animals. Trends Biochem Sci 27, 139-47.

Okumoto S., Schmidt R., Tegeder M., Fischer W.N., Rentsch D, Frommer W.B., Koch W.  (2002) High affinity amino acid transporters specifically expressed in xylem parenchyma and developing seeds of Arabidopsis. J Biol Chem 277, 45338-45346.

LalondeS., TegederM., Thorne-Holst M., FrommerW.B. and Patrick J.W. (2003) Phloem loading and unloading of sugars and amino acids. Plant Cell Environ 26, 37-56.

Wipf D. , Benjdia M., Rikirsch E., Zimmermann S., Tegeder M., and Frommer W.B.  (2003) A Hebeloma cylindrosporum expression cDNA library for suppression cloning in yeast mutants, complementation of a yeast his4 mutant and EST analysis. Genome 46, 177-81.

KochW., KwartM., Laubner M. , HeinekeD., StranskyH., FrommerW.B. and Tegeder M. (2003) Reduced amino acid content in transgenic potato tubers due to antisense inhibition of the leaf H+/amino acid symporter StAAP1. Plant J 33, 211-220.

Pelissier H. C., Frerich A., Desimone M., Schumacher M., and Tegeder M. (2004) PvUPS1, an allantoin transporter in nodulated roots of French bean. Plant Physiol 134, 664-675.

Okumoto S., Koch W., Tegeder M. Fischer W.N., Biehl A., Leister D., Stierhof Y. and Frommer W.B. (2004) Root phloem-specific expression of the plasma membrane amino acid proton co-transporter AAP3. J Exp Bot, 55: 2155 – 2168.

Lee Y.-H. and Tegeder M. (2004) Selective expression of a novel high affinity transport system for acidic and neutral amino acids in the tapetum cells of Arabidopsis flowers. Plant J, 40, 60-74. COVER ILLUSTRATION.

 

Publications before 2000

Pickardt T., Schieder O., Saalbach I., Tegeder M., Machemahl F., Saalbach G., Kohn H. and Muentz K. (1994). Regeneration and transformation in the legume Vicia narbonensis. In: Oono K. (ed.). Plant Tissue and Gene Manipulation for Breeding and Formation of Phytochemicals. NIAR, Japan, pp 89-94.

Tegeder M., Gebhardt D., Schieder O. and Pickardt T. (1995). Thidiazuron-induced plant regeneration from protoplasts of Vicia faba cv. Mythos. Plant Cell Rep 15, 164-169.

Tegeder M., Schieder O. and Pickardt T. (1995). Breakthrough in the plant regeneration from protoplasts of Vicia faba and V. narbonensis. Current Issues in Plant and Cellullar Biology. Kluwer, London, pp 75-80.

Tegeder M., Kohn H., Nibbe M., Schieder O. and Pickardt T. (1996). Plant regeneration from protoplasts of Vicia narbonensis via somatic embryogenesis and shoot organogenesis. Plant Cell Rep 16, 22-25.

Zenkteler M., Tegeder M., and Schieder O. (1998). Embryological studies of reciprocal crosses between Vicia faba and Vicia narbonensis. Acta Soc Bot Pol 67, 37-43.

Ward J.M., Kühn C., Tegeder M., and Frommer W.B. (1997). Sucrose transport in plants. International Review of Cytology 178, 41-71.

Harrington G.N., Nussbaumer Y., Wang X.-D. Tegeder M., Offler C.E, Patrick J.W., Franceschi V.R. and Frommer W.B. (1997). Temporal and spatial expression of plasma membrane sucrose transport-related genes in cotyledons of Vicia faba L. Protoplasma 200, 35-50.

Harrington G.N., Franceschi V.R., Offler C.E., Patrick J.W., Tegeder M., Frommer W.B., Harper J.F. and Hitz W.D. (1997). Cell specific expression of three genes involved in plasma membrane sucrose transport in developing Vicia faba seed. Protoplasma 197, 160-173.

Fischer W.-N., André B.A., Rentsch D., Krolkiewicz S., Tegeder M., Breitkreuz K. and Frommer W.B. (1998). Amino acid transport in plants. Trends Biochem Sci 3, 188-95.

Montamat F., Tegeder M., Frommer W.B. and Delrot S. (1999) Cloning and expression of a pod-specific amino acid transporter from broad bean. Plant Mol Biol 41, 259-268.

Tegeder M., Wang X.-D, Frommer W.B, Offler C.E, and Patrick J.W. (1999). Sucrose transport into developing seeds of Pisum sativum L. Plant J 18, 151-61.